Conformational comparisons of Pasteurella multocida types B and E and structurally related capsular polysaccharides

Richardson, Nichole I. and Ravenscroft, Neil and Kuttel, Michelle M. (2023) Conformational comparisons of Pasteurella multocida types B and E and structurally related capsular polysaccharides, Glycobiology, 33, 745–754, Oxford University Press.

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Abstract

Pasteurella multocida, an encapsulated gram-negative bacterium, is a significant veterinary pathogen. The P. multocida is classified into 5 serogroups (A, B, D, E, and F) based on the bacterial capsular polysaccharide (CPS), which is important for virulence. Serogroups B and E are the primary causative agents of bovine hemorrhagic septicemia that is associated with significant yearly losses of livestock worldwide, primarily in low- and middle-income countries. The P. multocida disease is currently managed by whole-cell vaccination, albeit with limited efficacy. CPS is an attractive antigen target for an improved vaccine: CPS-based vaccines have proven highly effective against human bacterial diseases and could provide longer-term protection against P. multocida. The recently elucidated CPS repeat units of serogroups B and E both comprise a N-acetyl-β-D-mannosaminuronic acid/N-acetyl-β-D-glucosamine disaccharide backbone with β-D-fructofuranose (Fruf) side chain, but differ in their glycosidic linkages, and a glycine (Gly) side chain in serogroup B. Interestingly, the Haemophilus influenzae types e and d CPS have the same backbone residues. Here, comparative modeling of P. multocida serogroups B and E and H. influenzae types e and d CPS identifies a significant impact of small structural differences on both the chain conformation and the exposed potential antibody-binding epitopes (Ep). Further, Fruf and/or Gly side chains shield the immunogenic amino-sugar CPS backbone—a possible common strategy for immune evasion in both P. multocida and H. influenzae. As the lack of common epitopes suggests limited potential for cross-reactivity, a bivalent CPS-based vaccine may be necessary to provide adequate protection against P. multocida types B and E.

Item Type:	Journal article (paginated)
Uncontrolled Keywords:	antigen conformation, capsular polysaccharide, cross reactivity, molecular modeling, Pasteurella multocida
Subjects:	Applied computing > Life and medical sciences > Computational biology > Molecular structural biology Computing methodologies > Modeling and simulation > Simulation types and techniques > Molecular simulation Applied computing > Physical sciences and engineering > Chemistry
Alternate Locations:	https://doi.org/10.1093/glycob/cwad049
Date Deposited:	09 Nov 2023 10:04
Last Modified:	09 Nov 2023 10:04
URI:	https://pubs.cs.uct.ac.za/id/eprint/1624

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