Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
Ravenscroft, Neil, A. Omar, J. Hlozek, C. Edmonds-Smith, R. Follador, F. Serventi, M. M. Kuttel, P. Cescutti and A. Faridmoayer (2017) Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.. Carbohydrate Research 450:19-29.
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Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized. Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B. Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A). The structure of the serotype 23A capsular polysaccharide is: ->4)-b-D-Glcp-(1->3)-[[a-L-Rhap-(1->2)]-[Gro- (2->P->3)]-b-D-Galp-(1->4)]-b-L-Rhap-(1->. This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted b-Gal is linked to b-Rha as a side chain. This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus. Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-b-Gal to resonate in the a-anomeric region. The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal a-Rha:->4)-b-D-Glcp-(1->4)-[Gro-(2->P->3)]-b-D-Galp-(1->4)-b-L-Rhap-(1->. The immunodominant terminal a-Rha of 23F is more sterically crowded in 23A and absent in 23B. This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.
|EPrint Type:||Journal (Paginated)|
|Subjects:||J Computer Applications: J.3 LIFE AND MEDICAL SCIENCES|
I Computing Methodologies: I.6 SIMULATION AND MODELING
|Deposited By:||Kuttel, Michelle|
|Deposited On:||23 November 2017|